Mio Nishizono's Locked-Room Murder Case (西園美魚密室殺人事件 Nishizono Mio Misshitsu Satsujin Jiken) is a Blu-ray disc game that was released in the first volume of the Blu-ray of Little Busters! Refrain on January 29, 2014. The game is in a visual novel style similar to the original Little Busters!. It was produced by Visual Art's. The story was written by Leo Kashida and the art was made by Na-Ga.

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